Earticle

초록

영어

Oyster mushroom, Pleurotus ostreatus is bifactorial heterothallism. Single basidiospore isolates from fruiting bodies are homokaryotic and self-sterile. However, we found that homokaryons derived from some strains of P. ostreatus could develop fruiting bodies with two different types. One hundred and two isolates out of 155 monospore isolates formed fruiting bodies (65.8%). First group did not have only mature or sporulating fruiting bodies but also clamp connections, which initial isolate also did not present clamp connections (Abortive homokaryotic fruiting, AHF). Second group had developed fruiting bodies with clamp connections even though initial homokaryotic colony did not form clamp connections (Pseudo- homokaryotic fruiting, PHF). The mycelial colonies derived from PHF by tissue culture formed clamp connections, while mycelial colonies of AHF lacked them. We obtained 535 PHF and 79 AHF inter-strain hybrids among 8 strains of P. ostreatus by hyphal anastomosis. The fruiting body yield of PHF group is higher than that of AHF group in bottle cultivation. A preselection of single spore isolates for fertility would save labour in strain improvement of P. ostreatus.

목차

ABSTRACT
 재료 및 방법
  균주 및 배양
  단포자 분리, 교배형 검정
  단핵체 자실체 유도
  계통간 교잡과 교잡체 (hybrid) 선발 및 자실체 특성 검정
 결과 및 고찰
  단핵체의 자실체 유도
  임성체간 및 비임성체간 교잡체의 자실체 특성 비교
 적요
 인용문헌

저자

• 유영복 [ Young-Bok Yoo | 농촌진흥청 농업과학기술원 응용미생물과 ] Corresponding author
• 김인엽 [ In-Yeup Kim | 농촌진흥청 농업과학기술원 응용미생물과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 농업과학기술원 응용미생물과 ]
• 장갑열 [ Kab-Yeul Jang | 농촌진흥청 농업과학기술원 응용미생물과 ]
• 오세종 [ Se-Jong Oh | 농촌진흥청 농업과학기술원 응용미생물과 ]
• 전창성 [ Chang-Sung Jhune | 농촌진흥청 농업과학기술원 응용미생물과 ]

참고문헌