Ribosomal DNA의 ITS 부위에 대한 RFLP 분석에 의한 Phellinus baumii PMO-P4의 유전학적 특성
Genetic characterization of Phellinus baumii PMO-P4 by analyzing restriction fragment length polymorphisms of nuclear ribosomal DNA internal transcribed spacers (ITS)
PMO-P4, being cultivated as “Sanghwang”in Korea, was proved to be P. baumii based on ITS (internal transcribed spacer) sequencing and RFLP (Restriction Fragment Length Polymorphism) patterns along with some Phellinus species including P. linteus. The similaraty of ITS sequencing between PMO-P4 and other Phellinus species was given the range of 48.6%~72.2%, showing the highest homology from P. linteus and the lowest from P. gilvus
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ABSTRACT 서론 실험 재료 Total genomic DNA 분리 PCR 제조 및 증폭 Restriction Fragment Length Polymorphism (RFLP) 분석 rDNA ITS 부위의 염기서열 및 상동성분석 결과 및 고찰 ITS 영역의 염기서열 분석 ITS 영역의 RFLP분석 ITS 영역을 이용한 진흙버섯속 균류의 유연관계 분석 적요 참고문헌