Earticle

초록

영어

This study was carried out to screen the fruiting body formation-specific genes from the medicinal mushroom Cordyceps militaris. A cDNA synthesized using total RNA from 4 stages of mushroom development, mycelium, primordium, immature fruiting body and mature fruiting body. Differential expression gene screening was performed by DD- PCR(Differential Display Arbitrary Primer PCR) with cDNA, we sequenced partial 6 genes using pGEM cloning vector. The DNA Sequence of the six DD-PCR products derived from differentially expressed genes was compared to that in the GenBank database by using the NCBI BLAST search to identify similarities to known sequences. Sequence analysis showed that six of DD-PCR products have unknown sequence.

목차

ABSTRACT
 MATERIALS AND METHODS
  Strain and Culture condition
  Preparation of totalRNA
  Synthesis of cDNA
  Differential Display an Arbitrary Primer PCR
  Cloning of partial fragment
 RESULTS AND DISCUSSION
 적요
 REFERENCES

저자

• Bangung Yun [ 윤방웅 | Dept. Biotechnology, School of Biological Sciences and Technology ]
• Ki-Chul Chung [ 정기철 | Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, Korea ] Corresponding author

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