Earticle

초록

영어

To develop neohaplonts for Ganoderma breeding, protoplasts were isolated from dikaryotic mycelium and regenerated. Selection rate of neohaplonts varied between ASI7074, ASI7091, ASI7094, ASI7100 and ASI7115, showing 5.24% on the average. Auxotropic mutants from Ganoderma monokarions were recovered by UV irradiation on protoplasts. Protoplast survival rates were 1.9% ASI 7074, 0.17% ASI 7091, and zero percent ASI 7100 using 300 second irradiation. Four auxotrophic strains were recovered from 1,536 colonies screened that will be further utilized for protoplast fusion and transformation.

목차

ABSTRACT
 재료 및 방법
  공시균주 및 배지
  Neohaplonts 선발
  돌연변이 유발
  영양요구주의 선발 및 유전표지 확인
 결과 및 고찰
  Neohaplonts 선발
  돌연변이 유기
  영양요구주 확인
 적요
 감사의 글
 참고문헌

저자

• 김경수 [ Kyung-Soo Kim | 농민버섯연구소 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 농업과학기술원 응용미생물과 ] Corresponding author
• 최선규 [ Sun-Gyu Choi | 농촌진흥청 농업과학기술원 응용미생물과 ]
• 유창현 [ Chang-Hyun You | 농촌진흥청 농업과학기술원 응용미생물과 ]
• 고미석 [ Mi-Suk Ko | 경상대학교 농학과 ]
• 서건식 [ Geon-Sik Seo | 한국농업전문학교 ]

참고문헌