Earticle

초록

영어

Osteoarthritis is one of the commonest causes associated with age-related damage of articular cartilage. Non-steroidal anti-inflammatory drugs are commonly used in osteoarthritic patient. However, long term administration of these drugs results gastrointestinal disorders. Though, most studies have demonstrated in the past that bee venom has therapeutic effect on diseases related to inflammation and pains, but its anti-inflammatory properties have not been so far studied on inflamed chondrocytes (LPS induced) invitro. For the purpose, the study was carried out to determine the effect of bee venom on porcine articular chondrocyte cell using microarray. In this study, we found that 2,235 significantly associated gene (1,404 up-regulated genes and 831 down-regulated genes) that were expressed on inflamed and non inflamed chondrocytes during proliferation. Among the 1,404 up-regulated genes and 831 down-regulated genes, known genes were 372 and 237, respectively. On the other hand, bee venom significantly reduced expression of fetuin involved in acute inflammatory reaction. Our results suggest that this study could be useful database in gene expression profiling of chondrocyte cell treated with bee venom.

목차

ABSTRACT
 Ι. 서론
 Ⅱ. 재료 및 방법
  1. 관절 연골세포의 분리 및 배양
  2. 봉독이 연골세포에 미치는 영향, 배양 및 봉독 처리
  3. RNA추출 및 Microarry분석
 Ⅲ. 결론 및 고찰
 Ⅳ. 요약
 Ⅴ. 인용문헌

저자

• 박새롬 [ Sairom Park | 강원대학교 동물생명과학대학 ]
• 이영섭 [ Yeung-sub Lee | 강원대학교 동물생명과학대학 ]
• 김진우 [ Jin Woo Kim | 강원대학교 동물생명과학대학 ]
• 김 훈 [ Hun Kim | 강원대학교 동물생명과학대학 ]
• 최소영 [ So-young Choi | 강원대학교 동물생명과학대학 ]
• 이지연 [ Ji-yeon Lee | 강원대학교 동물생명과학대학 ]
• 나레시 쿠마 싱 [ Naresh Kumar Singh | 강원대학교 동물생명과학대학 ]
• 오재돈 [ Jae-Don Oh | 한경대학교 유전정보연구소 ]
• 이학교 [ Hak-Kyo Lee | 한경대학교 유전정보연구소 ]
• 이성진 [ Sung-Jin Lee1 | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌