Earticle

초록

영어

Stearoyl-CoA desaturase (SCD1) gene plays important role in fatty acid composition. In order to find marker-assisted selection (MAS) for improving the economic trait, this study was performed to identify the 878T>C single nucleotide polymorphism (SNP) on SCD1 gene. Three genotypes (CC, CT, and TT) were detected in 878T>C SNP of SCD1 gene from 103 Hanwoo population by polymorphism chain reaction-restriction fragment length polymorphism (PCR-RFLP) and economic traits were analyzed by general linear model. The frequency of allele C and T was 0.534 and 0.466, also the genotype frequency of CC, CT and TT was 0.252, 0.563, and 0.184, respectively in the Hanwoo population. The TT genotype of SCD1 gene showed a significantly higher measures (p<0.05) of carcass weight (CW) than CT, CC genotype. No significant association was detected between genotype and other economic trait (marbling, backfat thickness, and longissimus muscle area) in this study. The results revealed that SCD1 gene 878T>C SNP could be useful for effective MAS to increase the economic quality in Hanwoo population.

목차

ABSTRACT
 Ι. 서론
 II. 재료 및 방법
  1. 공시재료 및 genomic DNA 추출
  2. 공시재료의 DNA 동일성 검사
  3. PCR primer 설계 및 증폭
  4. SCD1 유전자 878T>C SNP의 유전자형 분석
  5. 통계분석
 III. 결과 및 고찰
  1. 한우에서 SCD1 유전자의 PCR-RFLP
  2. 한우 SCD1 유전자의 SNP와 한우 경제형질과의 관련성 분석
 IV. 요약
 사사
 V. 인용문헌

저자

• 박새롬 [ Sairom Park | 강원대학교 동물생명과학대학 ]
• 홍민욱 [ Min-wook Hong | 강원대학교 동물생명과학대학 ]
• 김훈 [ Hun Kim | 강원대학교 동물생명과학대학 ]
• 이승규 [ Seung-kyu Lee | 강원대학교 동물생명과학대학 ]
• 이영섭 [ Yeung-sub Lee | 강원대학교 동물생명과학대학 ]
• 김진우 [ Jin-woo Kim | 강원대학교 동물생명과학대학 ]
• 송영한 [ Young-Han Song | 강원대학교 동물생명과학대학 ]
• 김기범 [ Ki-Beom Kim | 축산물품질평가원 ]
• 오재돈 [ Jae-Don Oh | 한경대학교 생명공학과 ]
• 이학교 [ Hak-Kyo Lee | 한경대학교 생명공학과 ]
• 최정우 [ Jung-woo Choi | 캐나다 궬프대학교 가축유전개량센터 ]
• 이성진 [ Sung-Jin Lee | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌