Earticle

초록

영어

The Oct-4 (octamer-4), a member of the POU family transcription factor, is expressed in early mouse embryogenesis and in pluripotent embryonic stem (ES) lines. Oct-4 expression is thought to remain confined to the germline after gastrulation in the embryo. Therefore, the study was designed to, study the location of Oct-4 protein in the ovaries, placenta and testis of Korean native cattle (Hanwoo). Expression of Oct-4 mRNA in the ovaries and placenta of bovine was confirmed by RT-PCR and immunohistochemical analysis. Oct-4 was expressed in granulosa, thecal cells irrespective of the shape and size of follicles and endometerium of Korean native cattle (Hanwoo). Expression of Oct-4 was profound in all the tissues of Korean native cattle (Hanwoo) suggestung their role in them. Oct-4 localization and expression could contribute to further developmental studies in Korean native cattle (Hanwoo).

목차

ABSTRACT
 Ι. 서론
 Ⅱ. 재료 및 방법
  1. 공시 동물
  2. 공시 재료의 처리
  3. RNA 추출 및 RT-PCR
  4. 면역조직 염색법(immunohistochemistry)
 Ⅲ. 결과 및 고찰
  1. 한우의 태반, 난소 및 정소에서의 Oct-4 mRNA 발현
  2. 한우의 난소와 태반에서의 Oct-4 단백질 발현 양상
 Ⅳ. 요약
 사사
 Ⅴ. 인용문헌

저자

• 김현 [ Hyun Kim | 국립축산과학원 가축유전자원시험장 ]
• 최재혁 [ Jae-Hyuk Choi | 국립축산과학원 동물바이오공학과 ]
• 김성우 [ Sung-woo Kim | 국립축산과학원 가축유전자원시험장 ]
• 양보석 [ Bohsuk Yang | 국립축산과학원 가축유전자원시험장 ]
• 고응규 [ Yeoung-Gyu Ko | 국립축산과학원 가축유전자원시험장 ] Corresponding Author

참고문헌