Earticle

초록

영어

Myopalladin (MYPN) is an important expression gene associated with regulation of Z-line structure in muscle and maintains sarcomeric integrity. In this study, we investigated the association between MYPN A1795G SNP (single nucleotide polymorphism) and carcass traits (LMA, longissimus muscle area; CW, carcass weight; BF, backfat thickness; MS, marbling score) in Korean cattle. The MYPN A1795G SNP was genotyped in 212 steers and analyzed the associations with carcass traits by PCR-RFLP (Restriction fragment length polymorphism) method. The allele frequencies were 0.566 for G allele and 0.434 for A allele. And the genotype frequencies of GG, GA, and AA genotype were 32.1%, 49%, and 18.9%, respectively. Association analysis indicated that the A1795G SNP of MYPN gene showed a significant association with LMA (p<0.05). The steers with GG genotype had higher LMA than those with the genotypes AA. But no significant associations were observed in other carcass traits (CW, BF, MS). The steers with the GG genotype showed higher CW and BF than those with the genotypes AA and GA. These results suggest that the A1795G SNP of the MYPN gene is associated with LMA and may be useful for candidate marker-assisted selection to increase the levels of LMA in Korean cattle.

목차

ABSTRACT
 Ι. 서론
 Ⅱ. 재료 및 방법
  1. 공시재료
  2. 시료의 DNA 동일성 검정
  3. Genomic DNA의 분리 및 정제
  4. MYPN 유전자의 primer 설계 및 합성
  5. MYPN 유전자의 PCR 증폭
  6. PCR-RFLP 분석에 의한 MYPN A1795G SNP의 유전자형 확인
  7. 통계분석
 Ⅲ. 결과 및 고찰
 Ⅳ. 요약
 Ⅴ. 인용문헌

저자

• 홍민욱 [ Min-wook Hong | 강원대학교 동물생명과학대학 ]
• 김훈 [ Hun Kim | 강원대학교 동물생명과학대학 ]
• 박새롬 [ Sairom Park | 강원대학교 동물생명과학대학 ]
• 이승규 [ Seung-kyu Lee | 강원대학교 동물생명과학대학 ]
• 이영섭 [ Yeung-sub Lee | 강원대학교 동물생명과학대학 ]
• 김진우 [ Jin-woo Kim | 강원대학교 동물생명과학대학 ]
• 김기범 [ Ki-Beom Kim | 축산물품질평가원 ]
• 이선영 [ Sun-Young Lee | 한국마사회 ]
• 송영한 [ Young-Han Song | 강원대학교 동물생명과학대학 ]
• 이성진 [ Sung-Jin Lee | 강원대학교 동물생명과학대학 ] Corresponding Author

참고문헌