Earticle

초록

영어

This study is conducted to screen compounds affecting ruminal fermentation under in vitro incubation. Saponin, chitosan, metformin, zinc acetate, zinc chloride, zinc oxide, zinc sulfate, ascorbic acid, oil-coated ascorbic acid, nicotinic acid, eastern herb cocktail, charcoal extract and garlic sources (lyophilized and extract) were added to rumen culture fluid at 1.25% of substrate (100% timothy) volume at 24 h incubation. pH, total gas, ammonia, VFAs were measured. Ascorbic acid increased total gas production indicating fermentation level. All zinc compounds significantly decreased (p<0.05) although zinc sulfate increased propionate of volatile fatty acid (p<0.05). In following experiment, ascorbic acid, oil-coated ascorbic acid, garlic lyophilized, herb cocktail and zinc sulfate were added to rumen culture fluid at 2.5% of substrate with 3, 6, 12 and 24 h incubation. Zinc sulfate decreased both ruminal fermentation and VFAs production but ascorbic acid enhanced total gas production. Ascorbic acid increased fermentation regardless of supplement concentration although excessive zinc sulfate decreased fermentation. These results suggest that optimal level of trace compounds might affect ruminal fermentation in ruminant.

목차

ABSTRACT
 Ι. 서론
 Ⅱ. 재료 및 방법
  1. 공시축
  2. 공시 첨가물질 및 처리방법
  3. 반추위 미생물의 혼합배양
  4. 분석방법
  5. 통계분석
 Ⅲ. 결과 및 고찰
  1. 육질개선 후보물질의 in vitro 배양에서의 pH와 총 가스발생량 분석
  2. 육질개선 후보물질의 in vitro 배양에서의 암모니아 농도의 분석
  3. 육질개선 후보물질의 in vitro 배양에서의 휘발성지방산 농도의 분석
  4. 육질개선 후보물질의 고농도 첨가수준이 pH의 경시적 변화에 미치는 영향 분석
  5. 육질개선 후보물질의 고농도 첨가수준이 총가스발생량의 경시적 변화에 미치는 영향 분석
  6. 육질개선 후보물질의 고농도 첨가수준이 암모니아 농도의 경시적 변화에 미치는 영향 분석
  7. 육질개선 후보물질의 고농도 첨가수준이 VFA 농도의 경시적 변화에 미치는 영향 분석
 Ⅳ. 결론
 Ⅴ. 요약
 Ⅵ. 인용문헌

저자

• 양승학 [ Seung-Hak Yang | 농촌진흥청 국립축산과학원 ] Corresponding Author
• 조원모 [ Won-Mo Cho | 농촌진흥청 국립축산과학원 ]
• 이현준 [ Hyun-Jun Lee | 농촌진흥청 국립축산과학원 ]
• 김상범 [ Sang-Bum Kim | 농촌진흥청 국립축산과학원 ]
• 김현섭 [ Hyun-Sup Kim | 농촌진흥청 국립축산과학원 ]
• 조성백 [ Sung-Baek Cho | 농촌진흥청 국립축산과학원 ]
• 박규현 [ Kyu-Hyun Park | 농촌진흥청 국립축산과학원 ]
• 곽정훈 [ Jung-Hun Kwag | 농촌진흥청 국립축산과학원 ]
• 최동윤 [ Dong-Yoon Choi | 농촌진흥청 국립축산과학원 ]
• 이상철 [ Sang-Chul Lee | 농촌진흥청 국립축산과학원 ]

참고문헌