Earticle

초록

영어

Background: The use of the extracts from evening primrose seeds as a health functional food has been gradually increased. Therefore, the monitoring and screening process has been considerably required for its quality control. Objective: This study aimed to estimate the measurement uncertainty associated with determination of penta-o-galloyl β-D-glucose (PGG) in extracts from evening primrose seeds by high-performance liquid chromatography. Methods: The sources of measurement uncertainty was expressed in accordance with mathematical/statistical theories of GUM & EURACHEM Guide. The expanded uncertainty was calculated by using the relative standard uncertainty between analytical result and sources of uncertainty in measurement (sample weight, final volume, extraction volume, standard solution, matrix and instrument etc). Results: In the results of 95% confidence interval, the uncertainty in measurement was 10,253.34 ± 1,844.50 μg/kg (k = 2.0). Conclusion: In this study, it showed that the value of uncertainty in measurement for determination of PGG in extracts from evening primrose seeds by HPLC has about 18.0% influence on PGG contents of the analytical results. The results would be very useful for the monitoring and screening of evening primrose seeds marketed in Korea for its quality control as dietary supplement.

목차

ABSTRACT
 연구 방법
  실험재료
  표준용액의 조제
  시험용액의 조제
  기기분석 조건
  불확도 추정방법
  모델관계식 설정
  요인별 측정값과 표준불확도
  검량곡선의 불확도
  합성표준불확도
  확장불확도
 연구 결과
  시험용액 조제 시 불확도
  표준용액의 순도 및 분자량의 불확도
  표준용액 제조과정의 불확도
  검량곡선의 불확도
  시료의 반복측정에 따른 불확도
  회수율 및 정밀성의 불확도
  합성표준불확도 및 확장불확도
  불확도 요인의 상대기여도
 고찰 및 결론
 참고문헌

키워드

저자

• 박상욱 [ Sang-Wook Park | 조선대학교 대학원 식품의약학과 ]
• 방준석 [ Joon Seok Bang | 숙명여자대학교 임상약학대학원 ]
• 이원재 [ Wonjae Lee | 조선대학교 대학원 식품의약학과, 조선대학교 약학대학 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국임상약학회지 [Korean Journal of Clinical Pharmacy]

• 간기

  계간

• pISSN

  1226-6051

• 수록기간

  1991~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 518 DDC 615