Earticle

초록

영어

Forest tree species usually takes for long periods to be harvested and cultivated but spearmints are a good model system for woody plant because of reducing and shortening cultivation time. Spearmints are good model plants (Mentha species) for research about terpenoids production and industrial essential oil manufacture. Isopentenyl pyrophosphate isomerase (Iso) and limonene synthase (Limo) are the key enzymes of terpenoid biosynthesis pathway. Transgenic and wild spearmints (Mentha spicata, MS) were cultured in vitro and assessed for the essential oil contents. The content of essential oil of transgenic spearmint also was enhanced slightly depending on the target terpenoid genes. In an attempt to increase productivity of terpenoids further, salt and fungal elicitation strategy was adopted on transgenic Mentha spicata. The salt (800 mM NaCl) as abiotic and two fungi (Botrytis cinerea and Glomerella cingulata) as biotic were used for elicitors. In the absence of salt stress four terpenoids were detected from the spearmint extracts, all of them being monoterpenes. On the other hand, the transgenic (MSIso) extracts contained eleven terpenoids (10 monoterpenes and 1 phenylpropene) while transgenic (MSLimo) extracts contained seven monoterpenes. After 3 days of fungal infection, the resistance indices further increased to 4.38, 3.89 and 2.04 for wild type, MSIso and MSLimo, respectively. The salt and fungal elicitators proved beneficial towards modifying both the terpenoids profile and improvement in the composition of essential oil. These results have important applications for the large-scale production of essential oils and forest biotechnology with respect to spearmint.

목차

Abstract
 Introduction
 Materials and Methods
  Plant materials and construction of gene expression vectors
  Salt and fungal elicitation of spearmints
  Extraction and metabolite profiling of spearmint
  Statistical analysis
 Results
  Variation in terpenoid composition of transgenic spearmint under salt stress
  Variations in the composition of terpenoids in transgenic spearmint after fungal elicitation
 Discussion
 Acknowledgements
 References

키워드

저자

• Myung Suk Choi [ Department of Forest Environmental Resources & Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, ]
• Dong Jin Park [ Department of Forest Environmental Resources & Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea ]
• Hyun Jin Song [ Department of Forest Environmental Resources & Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea ]
• Ji Yun Min [ Sancheong Oriental Medicinal Herb Institute, Sancheong 666-831, Republic of Korea ]
• Seung Mi Kang [ Department of Forest Research, Gyeongsangnam-do Forest Environment Research Institute, Jinju 660-871, Republic of Korea ]
• Chong Kyu Lee [ Department of Forest Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea ]
• Kye Man Cho [ Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea ]
• Chandrakant Karigar [ Department of Studies & Research in Biochemistry, Tumkur University, Tumkur 572-103, India ]
• Ho Kyoung Kim [ Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 305-811, Republic of Korea ]
• Young Min Kang [ Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 305-811, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634